Current Challenges in Real-time PCR Diagnostics in Food Science

  1. David Rodríguez-Lázaro 3
  2. Nigel Cook 1
  3. Marta Hernández Pérez 2
  1. 1 Food and Environment Research Agency
    info

    Food and Environment Research Agency

    Londres, Reino Unido

  2. 2 Instituto Tecnológico Agrario de Castilla y León
    info

    Instituto Tecnológico Agrario de Castilla y León

    León, España

    ROR https://ror.org/01f7a6m90

  3. 3 Universidad de Burgos
    info

    Universidad de Burgos

    Burgos, España

    ROR https://ror.org/049da5t36

Buch:
Real-time PCR in food science: current technology and applications
  1. David Rodríguez-Lázaro (ed. lit.)

Verlag: Caister Academic Press

ISBN: 978-1-908230-15-7

Datum der Publikation: 2013

Seiten: 21-26

Art: Buch-Kapitel

Zusammenfassung

A principal consumer demand is a guarantee of the safety and quality of food. The presence of food-borne pathogens and their potential hazard, the use of genetically modified organisms (GMOs) in food production, and the correct labelling in foods suitable for vegetarians are among the subjects where society demands total transparency. The application of controls within the quality assessment programmes of the food industry is a way to satisfy these demands, and is necessary to ensure efficient analytical methodologies are possessed and correctly applied by the Food Sector. The use of real-time PCR has become a promising alternative approach in food diagnostics. It possesses a number of advantages over conventional culturing approaches, including rapidity, excellent analytical sensitivity and selectivity, and potential for quantification. However, the use of expensive equipment and reagents, the need for qualified personnel, and the lack of standardized protocols are impairing its practical implementation for food monitoring and control.