Determinación de la ploidía en progenies de Tagetes spp. mediante citómetro de flujo

  1. Miguel Ángel Serrato-Cruz 1
  2. Martha Hernández-Rodríguez 2
  3. Yves Savidan 2
  4. Nina M. Bárcenas-Ortega 3
  1. 1 Universidad Autónoma Chapingo
    info

    Universidad Autónoma Chapingo

    Texcoco, México

    ROR https://ror.org/04ctjby61

  2. 2 Centro Internacional para el Mejoramiento de Maíz y Trigo, México
  3. 3 Colegio de Postgraduados Montecillo
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Journal:
Agrociencia

ISSN: 1405-3195,  2521-9766

Year of publication: 2000

Volume: 34

Issue: 6

Pages: 735-740

Type: Article

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More publications in: Agrociencia

Abstract

Flow cytometry was used to determine ploidy levels of Tagetes progenies obtained from crosses within and among populations of T. erecta , T. patula , two ecotypes of T. tenuifolia , and two unidentified wild populations. Progenies obtained from within population crosses showed the expected ploidy levels of 2x and 4x, corresponding to DNA average indexes of 0.48 and 1.00, respectively. Progenies from crosses between diploid populations and a tetraploid male had a DNA average index of 0.72 indicating a 3x ploidy level. Most progenies from crosses between diploid populations were diploids, but crosses between diploid and tetraploid populations showed triploid progenies and other unexpected ploidies suggesting the possible occurrence of unreduced gametes, apomixis, haploidy, and chimerism phenomena.

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