Production of omega-3 fatty acids acylglycerides by lipase-catalyzed glycerolysis of sardine oil in different reaction media

  1. García Solaesa, Ángela
Supervised by:
  1. Teresa Sanz Díez Director
  2. Sagrario Beltrán Calvo Director

Defence university: Universidad de Burgos

Year of defence: 2017

Committee:
  1. Juan Francisco Rodríguez Romero Chair
  2. Óscar Benito Román Secretary
  3. Blanca Celidet Pérez de Lucani Committee member
  4. Tiziana Fornari Committee member
  5. Ana Alexandra Figueiredo Matias Committee member
Department:
  1. BIOTECNOLOGIA Y CIENCIA DE LOS ALIMENTOS

Type: Thesis

Teseo: 521793 DIALNET lock_openRIUBU editor

Abstract

Production of omega-3 polyunsaturated fatty acids (n-3 PUFA) concentrates is of great interest for pharmaceutical and food industries. It is well known that the main n-3 PUFA, EPA and DHA, have important health benefits. n-3 PUFA monoacylglycerides and diacylglycerides (n-3 MAG and DAG) present good bioavailability and oxidation stability. The lipase-catalyzed glycerolysis reaction is the best alternative to produce the n-3 MAG. The main drawback of this process is the immiscibility of the substrates. The use of tert-alcohols, surfactants, supercritical CO2 and a substrate emulsification process have been evaluated to improve the contact between sardine oil and glycerol to achieve high yields of the reaction products. Regarding oxidation state, immobilized commercial lipases have presented adsorption capacity of the oxidation products formed during the glycerolysis reaction. Furthermore, molecular distillation process has been also optimized to fractionate the reaction products and concentrate the MAG rich in n-3 PUFA.