Efecto protector de un producto de hollejo sobre la activación de NRF2 y la inhibición de NF-KB en células huvec

Abstract

Endothelial dysfunction is the principal mechanism involved in the development of hypertension. It is the result of an imbalance between the levels of nitric oxide (NO) and free radicals (ROS) in the vascular endothelium by oxidative stress. Several cellular signaling pathways regulate the levels of these two molecules. That include the transcription factors NF-kB and Nrf2. Grape polyphenols have modulators effects on these factors, which will provide good protective effects of vascular endothelial function. The present work was developed using a new seasoning patented by Research Group UBU. The evaluation of the functional effects of seasoning was carried out by in vitro simulation of partial or complete digestion with gastrointestinal and colonic fermentation. The antioxidant capacity was determined by the ABTS method and was evaluated its protective effect on endothelial cell line (HUVEC) submitted to oxidative stress with a high concentration of glucose. Cell viability was studied using the MTT assay and cell impedance. It was observed a cytoprotective effect of fractions of gastrointestinal digestion and colonic fermentation. Also, analysis of the expression of transcription factors (NF-kB and Nrf2) by real-time PCR showed that the product has fully digested have protective effects in cells under oxidative stress by modulating the expression of Nrf2 and NF-kB.